Host Lipid Acyl Chains
نویسنده
چکیده
The partition coefficient Kp was measured for a headgroup-labeled phospholipid (12:0,12:0)-N-(7-nitrobenz-2oxa-1,3-diazol-4-yl)-PE (12-NBD-PE), equilibrated between LUV of a series of phosphatidylcholines (PC). Fluorescence resonance energy transfer between the 12-NBD-PE and a headgroup-rhodamine-labeled PE was used to find the equilibrium concentration of the 12-NBD-PE in the different LUV. Reliable equilibrium concentrations were obtained by monitoring the approach to equilibrium starting from a concentration below and from a concentration above the ultimate values. Using (16:0,18:1 A9)-PC as the reference lipid, Kp ranged from a high value of 1.65 favoring (16:0,18:1 A9)-PC over (16:1 A9,16:1 A9)PC, to a low value of 0.90, favoring (22:11A 3,22:1A13)-PC over (16:0,18:1A9)-PC. The Kp values enabled calculation of the acyl chain contribution to the excess free energy of mixing for (1 2:0,12:0) acyl chains at infinite dilution in the Laf phase of PC having acyl chains of (16:0,18:1 A9), (16:1 A9,16:1 A9), (18:1 A9,18:1 A9), (18:1 A6,18:1 A6), (20:1 Al 1,20:1 Al 1), and (22:1 Al 3,22: 1 Al 3). (14:1 A9,14:1 A9)-PC was found to transfer so rapidly between LUV as to preclude reliable Kp measurement.
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